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tritc conjugated pisum sativum agglutinin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories tritc conjugated pisum sativum agglutinin
    Tritc Conjugated Pisum Sativum Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1050-10/Unconjugated+Pisum+Sativum+Agglutinin+(PSA)/pm40806364-125-0-5
    Average 93 stars, based on 77 article reviews
    tritc conjugated pisum sativum agglutinin - by Bioz Stars, 2026-11
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    Related Articles

    Staining:

    Article Title: Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida
    Article Snippet: Post-incubation, the sperm were washed with 50 mM PBS pH 7.4, assessed for sperm viability by one step eosin-nigrosin staining method [ ] and 20 μl aliquots were spotted on poly-L-Lysine coated slides (Sigma-Aldrich Inc.) in duplicates. .. The spots were air-dried, fixed in chilled methanol for 30 seconds and stained with 5 μg/ml tetramethylrhodamine isothiocyanate conjugated Pisum sativum agglutinin (TRITC-PSA; Vector Laboratories Inc., Burlingame, CA, USA) for 30 min at RT. ..

    Article Title: Chronological age enhances aging phenomena and protein nitration in oocyte
    Article Snippet: .. Briefly, oocytes were subjected to acidified Tyrode’s solution to remove ZP and were fixed in 4% paraformaldehyde PFA at 37°C for 45 minutes, permeabilized using PBS Triton X-100 with 0.3% BSA for 45 minutes and blocked using 3% blocking solution overnight at 4°C and subsequently stained with primary anti α-tubulin antibody and an FITC conjugated secondary antibody as well as rhodamine conjugated pisum sativum agglutinin (PSA), and loaded in chambers containing Vectashield containing DAPI (Vector Laboratories, Eugene, OR) ( ). ..

    Blocking Assay:

    Article Title: Chronological age enhances aging phenomena and protein nitration in oocyte
    Article Snippet: .. Briefly, oocytes were subjected to acidified Tyrode’s solution to remove ZP and were fixed in 4% paraformaldehyde PFA at 37°C for 45 minutes, permeabilized using PBS Triton X-100 with 0.3% BSA for 45 minutes and blocked using 3% blocking solution overnight at 4°C and subsequently stained with primary anti α-tubulin antibody and an FITC conjugated secondary antibody as well as rhodamine conjugated pisum sativum agglutinin (PSA), and loaded in chambers containing Vectashield containing DAPI (Vector Laboratories, Eugene, OR) ( ). ..

    Binding Assay:

    Article Title: 'ZP domain' of human zona pellucida glycoprotein-1 binds to human spermatozoa and induces acrosomal exocytosis
    Article Snippet: .. Post-incubation, sperm were processed for simultaneous assessment of the status of acrosome by double labelling with 5 μg/ml tetramethyl rhodamine isothiocyanate conjugated Pisum sativum agglutinin (TRITC-PSA; Vector Laboratories Inc., Burlingame, CA, USA) and for binding of FITC-labelled human ZP1 273-551aa as described earlier [ ]. ..

    SPR Assay:

    Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker
    Article Snippet: All SPR procedures, i.e., pH immobilisation-scouting (using acetate buffers with pH 4.0, 4.5, 5 and 5.5), amine-coupling (using EDC, NHS and ethanolamine solutions) and single-cycle kinetics (SCK) analyses (kinetic titrations, including chip regeneration using 10 mM glycine HCl, pH 2.5) were performed using kits supplied by GE Healthcare (Vienna, Austria). .. The following unconjugated forms of lectins for the SPR analysis were provided by Vector Laboratories (USA): Aleuria aurantia lectin (AAL, L-1390), Galanthus nivalis lectin (GNL, L-1240), Maackia amurensis agglutinin II (MAAII, L-1260), Phaseolus vulgaris erythroagglutinin (PHA-E, L-1120), Phaseolus vulgaris leucoagglutinin (PHA-L, L-1110), Pisum sativum agglutinin (PSA, L-1050), Sambucus nigra agglutinin (SNA, L-1300) and Wisteria floribunda agglutinin (WFL, L-1350). .. To regenerate the biorecognition surface and remove the attached lectins, elution solutions from Vector Laboratories (Burlingame, CA, USA) were purchased, i.e., glycoprotein-eluting solution for mannose/glucose binding lectins (ES-1100-100); glycoprotein-eluting solution for galactose/GalNAc binding lectins (ES-2100-100); glycoprotein-eluting solution for fucose/arabinose binding lectins (ES3100-100); glycoprotein eluting solution for GlcNAc/Chitin binding lectins (ES-5100-100); glycoprotein-eluting solution for sialic acid binding lectins (ES-7100-100).

    other:

    Article Title: The sweeter aspects of platelet activation: A lectin-based assay reveals agonist-specific glycosylation patterns.
    Article Snippet: 20 Article history: 21 22 23 24 25 26 27 28 29 30 E D P R Received 14 May 2014 Received in revised form 17 August 2014 Accepted 21 August 2014 Available online xxxx



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    Profile of lectins binding to baculovirus-expressed human ZP1 273-551aa in ELISA . Microtitration plates were coated with the baculovirus-expressed recombinant ZP1 273-551aa (open bar; 500 ng/well) and processed for evaluation of binding to 21 different biotinylated lectins in an ELISA as described in Methods . As an internal control, microtitration plates coated with same amount of E. coli -expressed recombinant human ZP1 273-551aa (grey bar; unpublished observations) were also used. Values are expressed as absorbance obtained with various lectins binding to the respective recombinant protein, after deducting the non-specific binding of the lectins to the uncoated wells. Each bar represents a mean of duplicate experiments and standard deviation of the absorbance values. The lectins tested were GSL-I: Griffon simplicifolia lectin I, SBA: Soybean agglutinin, DSL: Datura stramonium lectin, PSA: <t>Pisum</t> <t>sativum</t> agglutinin, ConA: Concanavalin A, ECL: Erythrina cristagalli lectin, LCA: Lens culinaris agglutinin, DBA: Dolichos biflorus agglutinin, LEL: Lycopersicon esculentum lectin, PHA-L: Phaseolus vulgaris leucoagglutinin, UEA-I: Ulex europaeus agglutinin I, STL: Solanum tuberosum lectin, PHA-E: Phaseolus vulgaris erythroagglutinin, RCA: Ricinus communis agglutinin, VVA: Vicia villosa agglutinin, SJA: Sophora japonica agglutinin, PNA: Peanut agglutinin, S- WGA: Succinylated Wheat germ agglutinin, Jacalin, WGA: Wheat Germ agglutinin and GSL II: Griffonia simplicifolia lectin II.
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    Semen samples were pre-incubated with HHV-6B for one hr followed by incubation with either vehicle or calcium ionophore for 30 min to induce an acrosome reaction (AR). After incubation with both calcium ionophore and virus the samples were washed twice in PBS and stained with anti-gp60/110 or anti-IgG 2b followed by a goat-anti mouse (GaM)-FITC concomitantly with rhodamine-conjugated <t>pisum</t> <t>sativum</t> <t>(PSA).</t> The PSA (red) stains intact acrosomes and leaves acrosome reacted sperm unstained. (A-E) Different combinations of pre-treatment and subsequent staining are indicated at the bottom of the figure.
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    Image Search Results


    Summary of results obtained using lectins to bind (+) or not to bind (−) to exosomes immobilised at SPR sensor 1 chip.

    Journal: Sensors (Basel, Switzerland)

    Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker

    doi: 10.3390/s24041128

    Figure Lengend Snippet: Summary of results obtained using lectins to bind (+) or not to bind (−) to exosomes immobilised at SPR sensor 1 chip.

    Article Snippet: The following unconjugated forms of lectins for the SPR analysis were provided by Vector Laboratories (USA): Aleuria aurantia lectin (AAL, L-1390), Galanthus nivalis lectin (GNL, L-1240), Maackia amurensis agglutinin II (MAAII, L-1260), Phaseolus vulgaris erythroagglutinin (PHA-E, L-1120), Phaseolus vulgaris leucoagglutinin (PHA-L, L-1110), Pisum sativum agglutinin (PSA, L-1050), Sambucus nigra agglutinin (SNA, L-1300) and Wisteria floribunda agglutinin (WFL, L-1350).

    Techniques: Control

    Profile of lectins binding to baculovirus-expressed human ZP1 273-551aa in ELISA . Microtitration plates were coated with the baculovirus-expressed recombinant ZP1 273-551aa (open bar; 500 ng/well) and processed for evaluation of binding to 21 different biotinylated lectins in an ELISA as described in Methods . As an internal control, microtitration plates coated with same amount of E. coli -expressed recombinant human ZP1 273-551aa (grey bar; unpublished observations) were also used. Values are expressed as absorbance obtained with various lectins binding to the respective recombinant protein, after deducting the non-specific binding of the lectins to the uncoated wells. Each bar represents a mean of duplicate experiments and standard deviation of the absorbance values. The lectins tested were GSL-I: Griffon simplicifolia lectin I, SBA: Soybean agglutinin, DSL: Datura stramonium lectin, PSA: Pisum sativum agglutinin, ConA: Concanavalin A, ECL: Erythrina cristagalli lectin, LCA: Lens culinaris agglutinin, DBA: Dolichos biflorus agglutinin, LEL: Lycopersicon esculentum lectin, PHA-L: Phaseolus vulgaris leucoagglutinin, UEA-I: Ulex europaeus agglutinin I, STL: Solanum tuberosum lectin, PHA-E: Phaseolus vulgaris erythroagglutinin, RCA: Ricinus communis agglutinin, VVA: Vicia villosa agglutinin, SJA: Sophora japonica agglutinin, PNA: Peanut agglutinin, S- WGA: Succinylated Wheat germ agglutinin, Jacalin, WGA: Wheat Germ agglutinin and GSL II: Griffonia simplicifolia lectin II.

    Journal: Reproductive Biology and Endocrinology : RB&E

    Article Title: 'ZP domain' of human zona pellucida glycoprotein-1 binds to human spermatozoa and induces acrosomal exocytosis

    doi: 10.1186/1477-7827-8-110

    Figure Lengend Snippet: Profile of lectins binding to baculovirus-expressed human ZP1 273-551aa in ELISA . Microtitration plates were coated with the baculovirus-expressed recombinant ZP1 273-551aa (open bar; 500 ng/well) and processed for evaluation of binding to 21 different biotinylated lectins in an ELISA as described in Methods . As an internal control, microtitration plates coated with same amount of E. coli -expressed recombinant human ZP1 273-551aa (grey bar; unpublished observations) were also used. Values are expressed as absorbance obtained with various lectins binding to the respective recombinant protein, after deducting the non-specific binding of the lectins to the uncoated wells. Each bar represents a mean of duplicate experiments and standard deviation of the absorbance values. The lectins tested were GSL-I: Griffon simplicifolia lectin I, SBA: Soybean agglutinin, DSL: Datura stramonium lectin, PSA: Pisum sativum agglutinin, ConA: Concanavalin A, ECL: Erythrina cristagalli lectin, LCA: Lens culinaris agglutinin, DBA: Dolichos biflorus agglutinin, LEL: Lycopersicon esculentum lectin, PHA-L: Phaseolus vulgaris leucoagglutinin, UEA-I: Ulex europaeus agglutinin I, STL: Solanum tuberosum lectin, PHA-E: Phaseolus vulgaris erythroagglutinin, RCA: Ricinus communis agglutinin, VVA: Vicia villosa agglutinin, SJA: Sophora japonica agglutinin, PNA: Peanut agglutinin, S- WGA: Succinylated Wheat germ agglutinin, Jacalin, WGA: Wheat Germ agglutinin and GSL II: Griffonia simplicifolia lectin II.

    Article Snippet: Post-incubation, sperm were processed for simultaneous assessment of the status of acrosome by double labelling with 5 μg/ml tetramethyl rhodamine isothiocyanate conjugated Pisum sativum agglutinin (TRITC-PSA; Vector Laboratories Inc., Burlingame, CA, USA) and for binding of FITC-labelled human ZP1 273-551aa as described earlier [ ].

    Techniques: Binding Assay, Enzyme-linked Immunosorbent Assay, Recombinant, Standard Deviation

    Semen samples were pre-incubated with HHV-6B for one hr followed by incubation with either vehicle or calcium ionophore for 30 min to induce an acrosome reaction (AR). After incubation with both calcium ionophore and virus the samples were washed twice in PBS and stained with anti-gp60/110 or anti-IgG 2b followed by a goat-anti mouse (GaM)-FITC concomitantly with rhodamine-conjugated pisum sativum (PSA). The PSA (red) stains intact acrosomes and leaves acrosome reacted sperm unstained. (A-E) Different combinations of pre-treatment and subsequent staining are indicated at the bottom of the figure.

    Journal: PLoS ONE

    Article Title: Human Herpesvirus-6A/B Binds to Spermatozoa Acrosome and Is the Most Prevalent Herpesvirus in Semen from Sperm Donors

    doi: 10.1371/journal.pone.0048810

    Figure Lengend Snippet: Semen samples were pre-incubated with HHV-6B for one hr followed by incubation with either vehicle or calcium ionophore for 30 min to induce an acrosome reaction (AR). After incubation with both calcium ionophore and virus the samples were washed twice in PBS and stained with anti-gp60/110 or anti-IgG 2b followed by a goat-anti mouse (GaM)-FITC concomitantly with rhodamine-conjugated pisum sativum (PSA). The PSA (red) stains intact acrosomes and leaves acrosome reacted sperm unstained. (A-E) Different combinations of pre-treatment and subsequent staining are indicated at the bottom of the figure.

    Article Snippet: Samples were washed, stained and incubated with 1% paraformaldehyde as described above, only this time with co-staining using rhodamine-conjugated pisum sativum (PSA) (Vector Laboratories, Burlingame, CA, USA).

    Techniques: Incubation, Staining