tritc conjugated pisum sativum agglutinin (Vector Laboratories)
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Tritc Conjugated Pisum Sativum Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 77 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/L-1050-10/Unconjugated+Pisum+Sativum+Agglutinin+(PSA)/pm40806364-125-0-5
Average 93 stars, based on 77 article reviews
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Staining:Article Title: Delineation of downstream signalling components during acrosome reaction mediated by heat solubilized human zona pellucida Article Snippet: Post-incubation, the sperm were washed with 50 mM PBS pH 7.4, assessed for sperm viability by one step eosin-nigrosin staining method [ ] and 20 μl aliquots were spotted on poly-L-Lysine coated slides (Sigma-Aldrich Inc.) in duplicates. .. The spots were air-dried, fixed in chilled methanol for 30 seconds and stained with 5 μg/ml Article Title: Chronological age enhances aging phenomena and protein nitration in oocyte Article Snippet: .. Briefly, oocytes were subjected to acidified Tyrode’s solution to remove ZP and were fixed in 4% paraformaldehyde PFA at 37°C for 45 minutes, permeabilized using PBS Triton X-100 with 0.3% BSA for 45 minutes and blocked using 3% blocking solution overnight at 4°C and subsequently stained with primary anti α-tubulin antibody and an FITC conjugated secondary antibody as well as Blocking Assay:Article Title: Chronological age enhances aging phenomena and protein nitration in oocyte Article Snippet: .. Briefly, oocytes were subjected to acidified Tyrode’s solution to remove ZP and were fixed in 4% paraformaldehyde PFA at 37°C for 45 minutes, permeabilized using PBS Triton X-100 with 0.3% BSA for 45 minutes and blocked using 3% blocking solution overnight at 4°C and subsequently stained with primary anti α-tubulin antibody and an FITC conjugated secondary antibody as well as Binding Assay:Article Title: 'ZP domain' of human zona pellucida glycoprotein-1 binds to human spermatozoa and induces acrosomal exocytosis Article Snippet: .. Post-incubation, sperm were processed for simultaneous assessment of the status of acrosome by double labelling with 5 μg/ml SPR Assay:Article Title: Negative Charge-Carrying Glycans Attached to Exosomes as Novel Liquid Biopsy Marker Article Snippet: All SPR procedures, i.e., pH immobilisation-scouting (using acetate buffers with pH 4.0, 4.5, 5 and 5.5), amine-coupling (using EDC, NHS and ethanolamine solutions) and single-cycle kinetics (SCK) analyses (kinetic titrations, including chip regeneration using 10 mM glycine HCl, pH 2.5) were performed using kits supplied by GE Healthcare (Vienna, Austria). .. The following unconjugated forms of lectins for the SPR analysis were provided by Vector Laboratories (USA): Aleuria aurantia lectin (AAL, L-1390), Galanthus nivalis lectin (GNL, L-1240), Maackia amurensis agglutinin II (MAAII, L-1260), Phaseolus vulgaris erythroagglutinin (PHA-E, L-1120), Phaseolus vulgaris leucoagglutinin (PHA-L, L-1110), other:Article Title: The sweeter aspects of platelet activation: A lectin-based assay reveals agonist-specific glycosylation patterns. Article Snippet: 20 Article history: 21 22 23 24 25 26 27 28 29 30 E D P R Received 14 May 2014 Received in revised form 17 August 2014 Accepted 21 August 2014 Available online xxxx |


